Genotypes using Cotyledonary Node Explants
Journal of Plant Biochemistry & Physiology is handling volume:8, issue:2, 2020. Journal is focused on articles related to Plant Biosystems, Plant Ecology, Plant production science, Plant signaling & behavior, bacteriology, nematology, plant neurobiology.
A reproducible regeneration protocol for ICGV 12991, CG 7 and Red Valencia groundnut genotypes using Cotyledonary Node explants has been optimized. The effect of different BAP concentrations combined with either 2,4-D or TDZ was tested to determine optimum conditions for high shoot induction. Different BAP concentrations were tested to determine an optimum concentration for shoot elongation. Different NAA concentrations were similarly evaluated to determine the best concentration for rooting. Media containing combination of 5 mg/L BAP and 1 mg/L TDZ was the best concentration for shoot induction while media containing BAP at 5 mg/L was the best for elongation of shoots. NAA concentration of 1 mg/L gave the highest number of plants with roots. This works provides a very good protocol which will be beneficial during groundnut tissue culture as well as genetic transformation of groundnuts.
Нe rooting of primary regenerants was optimized by using dLوٴerent concentrations of NAA (0.5, 1, 1.5 and 2 mg/L). All the concentrations in all the genotypes produced roots, but 1 mg/L produced the best roots within 21 days aіer transfer into root induction media. Similar results were observed for genotypes PBS24030, HNG-10, M-13 and M-335 . A high NAA concentration (6 mg/L) was found to aوٴect initiation of roots in all the genotypes. Read the full article on journal website.
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Journal of Plant Biochemistry and Physiology